Hoefer HE-PLUS System Bedienungsanleitung

user manualum  HE-PLUS/Rev. B0/07-12Hoefer HE-PLUS Horizontal Gel Electrophoresis System

• pixSpecifications Unit dimensions (W × L × H)  24.5 × 17.0 × 6.2 cmGel dimensions (W × L)  12.5 × 13.0 cm Maximum sample capacity   112 samples (4 c

• p1Operating InstructionsA. Guidelines  for Selecting Electrophoresis Buffers and  Gel ConcentrationsThe two most commonly used buffers for hori-zont

• p21Make 500 ml of either 1X TAE or 1X TBE electrophoresis buffer.2Weigh an appropriate quantity of  agarose (see  Table 1) and place it into a 250 m

• p33Make note of the total solution volume so that degree of evaporation can be determined and corrected for.4Heat the agarose slurry in a microwave 

• p4B. Casting  the Gel1Place the gel casting stand on a lab bench. 2Insert the gel casting tray into the casting stand. If you are using the 6 × 12.5

• p55Insert one or more combs by placing them into the slots in the casting stand. For best results, place the comb in the slot nearest the end of the

• p6C. Removing  the Comb1When the gel is solidified and fully opaque, carefully remove the comb with a gentle wiggling, upward motion. If the comb is

• p7E. Electrical  Connections to the Safety Lid The Hoefer HE-PLUS Gel System can only be operated with the safety lid in place. Elec-trical current

• p8F. Setting the Power Supply1Ensure an approved power cord that satisfies your regional voltage standard is used. Input voltage is automatically de

• p9G. To Pause a Run and/or Change Parameters 1To pause the run select Pause. During the pause mode the voltage amperage or time can be changed by hi

• piContents Important Information ... iiWaste Electrical and  Electronic Equipment (WEEE) ...

• p10I. Detection  and Documentation of Separated Fragments1At the completion of the run, turn off the power supply and disconnect the power cord. Rem

• p11Troubleshooting GuideProblem Cause SolutionThe LCD screen remains blank and the fan does  not run when the power  is turned on.AC power cord is n

• p12Solutions Tris Acetate EDTA Buffer (TAE)1X Working Concentration:40 mM   Tris base20 mM   Glacial Acetic Acid (NaOAc) 2.0 mM   EDTA pH 8.3  10X S

• p13References1. Lehrach, H., et al. 1977. Biochemistry 16:4743.2. Sambrook, J., Fritsch, E.F., and Maniatis, T., (1989). Molecular Cloning, A Labo

• p14Ordering Information Product  Code No.HE-PLUS Electrophoresis  HE-PLUS-115V complete system 115 V.   Includes gel tank, safety lid,  power supply

• p15Care and MaintenanceCleaningUnits are best cleaned using warm water and a mild detergent. Water at temperatures above  60 °C can cause damage to 

Hoefer, Inc. 84 October Hill Road Holliston, MA 01746Toll Free: 1-800-227-4750 Phone: 1-508-893-8999 Fax: 1-508-893-0176 E-mail: [email protected]

• piiImportant Information  – English• If this equipment is used in a manner not specified by Hoefer, Inc. the protection provided by the equipment m

• piiitørken komplet før brug. • Driver ikke instrumentet i yderst fugtighed (ovenfor 80%). Undgå kondensation ved lade enheden equili-brate til omgi

• piv1:2001 norme de sécurité électrique. Néanmoins, il devrait être seulement utilisé par les opérateurs convenablement entraînés. Lire ce manuel ent

• pv• Non opera lo strumento nell’umidità estrema (al di sopra di 80%). Evitare la condensazione lasciando l’unità equilibra alla temperatura ambient

• pvisuaoperação. • Este instrumento é projectado de acordo com o EN61010-1:2001 condição de segurança eléctrica. Não obstante, deve ser usado só po

• pviiEnglishFrenchGermanItalianSpanishSwedishThis symbol indicates that the waste of electrical and electronic equipment must not be disposed as unso

• pviiiPacking List  Hoefer HE-PLUS Gel SystemUnits include:Tank with lid, power supply, power cord, and standard casting set (one – 13 × 12.5 cm UV T